9/7/2023 0 Comments What is cccp treated cells![]() LC3b, on the other hand, was induced and did not colocalize with mitochondria in SS conditions. Parkin co-localized with mitochondrial marker Tom20 after CCCP treatment, especially when cells were grown in CM. Mitochondrial function was determined by RT- PCR assay for mtDNA/genomic DNA ratio and ATP productionĬCCP induced mitophagy in cells grown in CM with significant Parkin induction (P<0.01) while SS treated cells exhibited no Parkin expression, but twice more LC3b conversion than CM treated cells (P< 0.05). Mitophagy was assessed by Western blot and confocal imaging of LC3b and Parkin. ARPE19 cells with intact or knocked down (KD) Pink1 were cultured in either complete medium (CM) or serum starved (SS) conditions followed by CCCP (1uM or 10uM) treatment for 4h or 24 h. Mitophagy is initiated by mitochondrial translocation of Pink1 with Parkin recruitment, followed by the general autophagy pathway including LC3b recruitment and lysosomal degradation. Serum-starvation can induce generalized autophagy whereas CCCP specifically induces mitophagy. This study focuses on importance of mitophagy in ARPE19 cells under acute stress Induction of mitophagy/autophagy may be a treatment strategy to prevent RPE degeneration during stress. Autophagic dysregulation have been suggested in a variety of neurodegenerative diseases including age-related macular degeneration (AMD), but mitophagy has not been studied in depth. Autophagy and mitophagy are protective mechanisms in cells, particularly Retinal Pigment epithelium (RPE), that are constantly under stress. ![]()
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